Scientist/Sr Scientist- Cell Engineering & Expression

Company: Zoetis
Location: Fort Collins
Posted on: May 3, 2021

Job Description:

Zoetis is seeking a talented and experienced scientific researcher to contribute to our efforts at the research incubator, Global Therapeutics Research in Fort Collins, CO. This individual will partner with project teams to develop and functionally characterize cell lines used to build a basic understanding of disease targets, pathways, and therapeutic mechanisms of action in addition to contributing to novel therapeutic platform development.

The successful candidate should have knowledge of molecular and cell biology aspects of functional cell line development from vector design/construction, gene editing, cell culture maintenance and transient transfections, clone screening, and functional characterization through protein assays. Experience with and understanding of immune cells, antibody function and CRISPR is highly desired. Working knowledge of methodologies to generate and grow hybridomas is a plus. Excellent communication, organizational and interpersonal skills are essential. Some travel required.

Please note: This position is for a fixed term of 3.5 years.

POSITION RESPONSIBILITIES:

• Design expression vectors/plasmids
• Generation and evaluation of reporter cell lines and/or knock out/knock in lines for pre-clinical development programs (genomic editing techniques such as CRISPR and/or shRNa)
• Mammalian expression of proteins in transient systems (HEK/CHO)
• Design and develop fit-for-purpose assays including molecular assays, immunoassays, high-sensitivity immunoassays for characterizing cell lines and developing biomarkers.
• Develop novel strategies for improving speed of cell line development and implement improvements to existing platform technologies.
• Collaborate across multiple scientific disciplines to understand mechanistic biology a
• Directly communicate with external partners for collaborations and outsourcing.
• Design fit-for-purpose quantitative metabolite assays and perform metabolite extractions to support mass spectrometric analyses.
• Design and execute bench level experiments, analyze and interpret data, and be able to clearly communicate the results of that work

EDUCATION AND EXPERIENCE:

• Degree in an applicable field (Cellular biology, Microbiology, Veterinary Science, Biochemistry, Biotechnology, Genetics, Engineering, Immunology or related field) with 7+ years of experience for Bachelor's, 5-7 years for Master's and 0+ years for PhD.
• High proficiency in mammalian cell culture and functional cell line development and experienced in a wide range of state-of-the-art molecular and cellular biology techniques (cloning, subcloning, qPCR, isothermal PCR, arrays etc.)
• Experience with genomic or genome editing approaches to assess gene function (CRISPR and/or shRNA)
• Deep knowledge of designing and developing immunoassays and optimizing methods and reagents for cellular readouts and protein characterization.
• High proficiency in cellular screening, experience with laboratory automation is a plus.
• Experience with hybridoma fusion technology is a plus.
• Experience in designing and developing diagnostic assays is highly preferred.
• Experience working with primary immune cells such as T cells, NK cells.
• Self-starter, able to prioritize work and work efficiently, design experiments with minimal supervision, and able work across teams remotely and face-to-face.
• Experience interacting with CROs or external partnerships
• Excellent verbal/written communication, organizational and interpersonal skills
• Computer skills required: Microsoft Word, Excel, Outlook, BLAST, Vector NTI or equivalent and NGS analysis software.

ADDITIONAL INFORMATION:

Work environment:

The placement of this position will be primarily in Fort Collins, CO. Occasional travel to R&D headquarters in Kalamazoo, MI may also be requested.

The person filling this position may work primarily in a laboratory setting. There may be exposure to animal pathogens and chemicals. There may be handling of, and/or exposure to animal tissue and zoonotic organisms

Zoetis is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status or any other protected classification. Zoetis also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as employment eligibility verification requirements of the Immigration and Nationality Act. All applicants must possess or obtain authorization to work in the US for Zoetis. Zoetis retains sole and exclusive discretion to pursue sponsorship for the acquisition or maintenance of nonimmigrant status and employment eligibility, considering factors such as availability of qualified US workers. Individuals requiring sponsorship must disclose this fact.

Full time

Fixed Term/Temporary (Fixed Term)

Colleague

Zoetis is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status or any other protected classification. Disabled individuals are given an equal opportunity to use our online application system. We offer reasonable accommodations as an alternative if requested by an individual with a disability. Please contact Zoetis Colleague Services at zoetiscolleagueservices@zoetis.com to request an accommodation. Zoetis also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as employment eligibility verification requirements of the Immigration and Nationality Act. All applicants must possess or obtain authorization to work in the US for Zoetis. Zoetis retains sole and exclusive discretion to pursue sponsorship for the acquisition or maintenance of nonimmigrant status and employment eligibility, considering factors such as availability of qualified US workers. Individuals requiring sponsorship must disclose this fact. Please note that Zoetis seeks information related to job applications from candidates for jobs in the U.S. solely via the following: (1) our company website at www.Zoetis.com/careers site, or (2) via email to/from addresses using only the Zoetis domain of "@zoetis.com". In addition, Zoetis does not use Google Hangout for any recruitment related activities. Any solicitation or request for information related to job applications with Zoetis via any other means and/or utilizing email addresses with any other domain should be disregarded. In addition, Zoetis will never ask candidates to make any type of personal financial investment related to gaining employment with Zoetis.

Keywords: Zoetis, Fort Collins , Scientist/Sr Scientist- Cell Engineering & Expression, Other , Fort Collins, Colorado